Publications
* Co-authors / ‡ Co-corresponding authors
2024
Chen S, Basiashvili T, Hutchings J, Murillo MS, Suarez AV, Louro JA, Leschziner AL‡, Villa E‡ (2024). Cryo-electron tomography reveals the microtubule-bound form of inactive LRRK2. bioRxiv.
Dederer V*, Sanz Murillo M*, Karasmanis EP*, Hatch KS, Chatterjee D, Preuss F, Abdul Azeez KR, Nguyen LV, Galicia C, Dreier B, Plückthun A, Versees W, Mathea S, Leschziner AE, Reck-Peterson SL‡, Knapp S‡ (2024). A designed ankyrin-repeat protein that targets Parkinson’s disease-associated LRRK2. J Biol Chem 300(7). (Here is the bioRxiv version.)
Sarsam RD*, Xu J*, Lahiri I, Gong W, Oh J, Zhou Z, Chong J, Hao N, Li S, Wang D‡, and Leschziner AE‡ (2024). Elf1 promotes Rad26’s interaction with lesion-arrested Pol II for transcription-coupled repair. Proc Natl Acad Sci USA 121(3). (Here is the bioRxiv version.)
2023
Reimer JM*, Dickey AM*, Lin YX*, Abrisch RG, Mathea S, Chatterjee D, Fay EJ, Knapp S, Daugherty MD, Reck-Peterson SL‡, Leschziner AE‡ (2023). Structure of LRRK1 and mechanisms of autoinhibition and activation. Nat Struct Mol Biol (Online ahead of print).
Karasmanis EP*, Reimer JM*, Kendrick AA*, Nguyen KHV, Rodriguez JA, Truong JB, Lahiri I, Reck-Peterson SL‡, Leschziner AE‡ (2023). Lis1 relieves cytoplasmic dynein-1 autoinhibition by acting as a molecular wedge. Nat Struct Mol Biol 30(9):1357.
Murillo MS*, Suarez AV*, Dederer V*, Chatterjee D, Louro JA, Knapp S, Mathea S, Leschziner A (2023). Inhibition of Parkinson’s Disease-related LRRK2 by type-I and type-II kinase inhibitors: activity and structures. Sci Adv 9(48). (Here is the bioRxiv version.)
Reimer JM, DeSantis ME, Reck-Peterson SL, Leschziner AE (2022). Structures of human cytoplasmic dynein in complex with the lissencephaly 1 protein, LIS1. eLIFE:e84302 (Here is the bioRxiv version.)
2022
Snead DM*, Matyszewski M*, Dickey AM*, Lin YX, Leschziner AE‡, Reck-Peterson SL‡ (2022). Structural basis for Parkinson’s Disease-linked LRRK2’s binding to microtubules. Nat Struct Mol Biol 29:1196. (Here is the bioRxiv version.)
Reimer JM, Dickey AM*, Lin YX*, Abrisch RG*, Mathea S, Chatterjee D, Fay EJ, Daugherty MD, Reck-Peterson SL‡, Leschziner AE‡ (2022). Structure of LRRK1 and mechanisms of autoinhibition and activation. bioRxiv
Karasmanis EP*, Reimer EM*, Kendrick AA*, Rodriguez JA, Truong JB, Lahiri I, Reck-Peterson SL‡, Leschziner AE‡ (2022). Lis1 relieves cytoplasmic dynein-1 auto-inhibition by acting as a molecular wedge. bioRxiv
Gillies JP*, Reimer JM*, Karasmanis EP*, Lahiri I, Htet ZM, Leschziner AE‡, Reck-Peterson SL‡ (2022). Structural basis for cytoplasmic dynein-1 regulation by Lis1. eLIFE 11:e71229
2021
Leschziner AE, Reck-Peterson SL (2021). Scientific Perspectives: Structural biology of LRRK2 and its interaction with microtubules. Mov Disord 36(11):2494
Sharon I, Haque AS, Grogg M, Lahiri I, Seebach D, Leschziner AE, Hilvert D, Schmeing TM (2021). Structures and function of the amino acid polymerase cyanophycin synthetase. Nat Chem Biol 17(10):1101
Baker RW*, Reimer JM*, Carman PJ, Turegun B, Arakawa T, Dominguez R, Leschziner AE (2021) Structural insights into assembly and function of the RSC chromatin remodeling complex. Nat Struct Mol Biol 28(1):71
2020
Deniston C*, Salogiannis J*, Mathea S*, Snead DM, Lahiri I, Matyszewski M, Donosa O, Watanabe R, Bohning J, Shiau AK, Knapp S, Villa E, Reck-Peterson SL‡, Leschziner AE‡ (2020) Structure of LRRK2 in Parkinson’s Disease and model for microtubule interaction. Nature 588(7837):344
Htet ZM*, Gillies JP*, Baker RW, Leschziner AE, DeSantis ME‡, Reck-Peterson SL‡ (2020) LIS1 promotes the formation of activated cytoplasmic dynein-1 complexes. Nat Cell Biol 22(5):518
Xu J, Wang W, Xu L, Chen J-Y, Chong J, Oh J, Leschziner AE, Fu X-D, Wang D (2020) Cockayne syndrome B protein acts as an ATP-dependent processivity factor that helps RNA polymerase II overcome nucleosome barriers. Proc Nat Acad Sci USA 117(41):25486
Lumpkin RJ*, Baker RW*, Leschziner AE, Komives EA (2020) Structure and dynamics of the ASB9 CUL-RING E3 Ligase. Nat Commun 11(1):2866
2019
Partlow EA*, Baker RW*‡, Beacham GM, Chappie JS, Leschziner AE‡, Hollopeter G‡ (2019) A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex. eLIFE 8:e50003
Lahiri I, Xu J, Han BG, Oh J, Wang D, DiMaio. F, Leschziner AE (2019) 3.1Å structure of yeast RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion solved using streptavidin affinity grids. J Struct Biol 207(3):270
2018
Cianfrocco MA‡, Lahiri I, DiMaio F, Leschziner AE (2018) cryoem-cloud tools: A software platform to deploy and manage cryo-EM jobs in the cloud. J Struct Biol 203(3):230
Turegun B, Baker RW, Leschziner AE and Dominguez R (2018) Actin-related proteins regulate the RSC chromatin remodeler by modulating intramolecular interactions of the Sth1 ATPase. Communications Biology 1:1
2017
DeSantis ME*, Cianfrocco MA*, Htet ZM*, Tran PT, Reck-Peterson SL‡ and Leschziner AE‡ (2017) Lis1 has two opposing modes of regulating cytoplasmic dynein. Cell 170(6): 1197
Xu J*, Lahiri I*, Wang W, Wier A, Cianfrocco MA, Chong J, Hare A, Dervan PB, DiMaio F, Leschziner AE‡ and Wang D‡ (2017) Structural basis for eukaryotic transcription-coupled DNA repair initiation. Nature 551(7682):653
Cui J, Zhu Q, Zhang H, Cianfrocco MA, Leschziner AE, Dixon JE and Xiao J (2017) Structure of Am20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding. eLIFE 6:e23990
2015
Cianfrocco MA*, DeSantis ME*, Leschziner AE and Reck-Peterson SL (2015). Mechanism and regulation of cytoplasmic dynein. Annu Rev Cell Dev Biol 31: 83-108.
Cianfrocco M and Leschziner AE (2015) Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud. eLIFE:06664
2014
Toropova K*, Zou S*, Roberts AJ, Redwine WB, Goodman BS, Reck-Peterson SL‡ and Leschziner AE‡ (2014). Lis1 regulates dynein by sterically blocking its mechanochemical cycle. eLIFE 3:e03372
Cianfrocco MA and Leschziner AE (2014). Traffic control: Adaptor proteins guide dynein-cargo takeoff. EMBO J, 33 (17):1845
2013
Nguyen VQ, Ranjan A, Stengel F, Wei D, Aebersold R, Wu C and Leschziner AE (2013). Molecular architecture of the ATP-dependent chromatin remodeling complex SWR1. Cell 154(6): 1220.
2012
Redwine WB*, Hernandez-Lopez RH*, Zou S, Huang J, Reck-Peterson SL and Leschziner AE (2012). Structural basis for microtubule binding and release by dynein. Science 337:1532
Huang J, Roberts AJ, Leschziner AE and Reck-Peterson SL (2012). Lis1 acts as a “clutch” between the ATPase and microtubule-binding domains of the dynein motor. Cell 150(5):975
Derr N, Goodman BS, Jungmann R, Leschziner AE, Shih W and Reck-Peterson SL (2012). Tug of war in motor protein ensembles revealed with a programmable DNA origami scaffold. Science 338: 662.
2011
Leschziner AE (2011). Electron microscopy studies of nucleosome remodelers. Curr Opin Struct Biol 21(6):709
Chandramouli P, Hernandez-Lopez R, Wang HW and Leschziner AE (2011). Validation of the Orthogonal Tilt Reconstruction method with a biological test sample. J Struct Biol 175(1):85
2010
Leschziner A. (2010). The orthogonal tilt reconstruction method. Methods Enzymol. 482:237
Norville JE, Derda R, Gupta S, Drinkwater KA, Belcher AM, Leschziner AE and Knight Jr. TF (2010). Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits. J Biol Eng 4:17
2009
Bleichert F, Gagnon KT, Brown BA 2nd, Maxwell ES, Leschziner AE, Unger VM and Baserga SJ (2009). A dimeric structure for archaeal box C/D small ribonucleoproteins. Science 325(5946):1384
Publications as postdoc
Wang HW, Ramey V, Westermann S, Leschziner AE, Welburn J, Drubin D, Barnes G and Nogales E (2007). Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nat Struct Mol Biol 14(8):721
Leschziner AE, Saha A, Wittmeyer J, Zhang Y, Bustamante C, Cairns BR and Nogales E (2007). Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method. Proc Natl Acad Sci USA 104(12): 4913
Leschziner AE and Nogales E (2007). Visualizing flexibility at molecular resolution: Analysis of heterogeneity in single-particle electron microscopy reconstructions. Annu Rev Biophys Biomol Struct Vol.36: 43
Leschziner AE and Nogales E (2006). The Orthogonal Tilt Reconstruction method: an approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles. J Struct Biol 153(3):284
Clarey MG, Erzberger JP, Grob P, Leschziner AE, Berger JM, Nogales E and Botchan M (2006). Nucleotide-dependent conformational changes in the DnaA-like core of ORC. Nat Struct Mol Biol 13(8):684
Leschziner AE, Lemon B, Tjian R and Nogales E (2005). Structural studies of the human PBAF chromatinremodeling complex. Structure (Camb) 13(2): 267
